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Arora, P. K.
- Development and Validation of HPTLC Method for the Estimation of Ondansetron Hydrochloride in Bulk Drug and Tablet Dosage Forms
Abstract Views :193 |
PDF Views:89
Authors
A. Chauhan
1,
P. K. Arora
2,
A. Yadav
2,
R. M. Singh
2,
D. K. Sharma
2,
S. C. Mathur
2,
G. N. Singh
2
Affiliations
1 Indian Pharmacopoeial Laboratory, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Govt, of India, Sector -23, Rajnagar, Ghaziabad - 201002, IN
2 Indian Pharmacopoeial Laboratory, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Govt, of India, Sector -23, Rajnagar, Ghaziabad- 201002, IN
1 Indian Pharmacopoeial Laboratory, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Govt, of India, Sector -23, Rajnagar, Ghaziabad - 201002, IN
2 Indian Pharmacopoeial Laboratory, Indian Pharmacopoeia Commission, Ministry of Health & Family Welfare, Govt, of India, Sector -23, Rajnagar, Ghaziabad- 201002, IN
Source
Journal of Pharmaceutical Research, Vol 12, No 2 (2013), Pagination: 61-65Abstract
A simple, selective, precise and sensitive high performance thin-layer chromatographic (HPTLC) method has been developed and validated for the analysis of ondansetron hydrochloride both in bulk drug and in tablet dosage forms. The separation was performed on pre-coated silica gel 60 GF254 plates using methanol:triethylamine:glacial acetic acid (9.5:0.5:0.1, v/v/v) as mobile phase. Densitometric analysis was performed in reflectance-absorbance mode at 309 nm. The linear regression analysis data for the calibration plot showed good linear relationship with R2 = 0.998 ± 0.00047 in the range of 300-1100 ng spot-1. The minimum amount of ondansetron hydrochloride that could be detected and quantified was 54.60 and 165.46 ng spot-1 respectively. The mean retardation factor (Rf) for ondansetron hydrochloride was found to be 0.77 ± 0.01. The developed method was validated according to International Conference on Harmonization (ICH) guidelines for specificity, linearity, range, accuracy, precision, detection limit, quantitation limit and robustness etc.Keywords
Ondansetron Hydrochloride, HPTLC, Method Validation.Full Text
- Seasonal Incidence of Mites on Kinnow in Punjab
Abstract Views :91 |
PDF Views:65
Authors
Affiliations
1 Krishi Vigyan Kendra, Gurdaspur 143521, Punjab, IN
2 Department of Entomology, Punjab Agricultural University, Ludhiana 141004, Punjab, IN
3 Regional Research Station, Abohar 152116, Punjab, IN
1 Krishi Vigyan Kendra, Gurdaspur 143521, Punjab, IN
2 Department of Entomology, Punjab Agricultural University, Ludhiana 141004, Punjab, IN
3 Regional Research Station, Abohar 152116, Punjab, IN
Source
Indian Journal of Entomology, Vol 84, No 2 (2022), Pagination: 356-359Abstract
Field surveys were conducted at ten days intervals from February 2016 to September 2017 in six Kinnow orchards at three locations (Ludhiana, Abohar, Hoshiarpur) in different agroclimatic zones of Punjab to evaluate the population dynamics of mites on kinnow. The study revealed the occurrence of Brevipalpus phoenicis (Geijskes), B. californicus (Banks), Tetranychus urticae Koch, Eutetranychus orientalis (Klein) and Polyphagotarsonemus latus (Banks) as the most common phytophagous mites. The predatory mites viz., Euseius alstoniae Gupta, E. finlandicus (Oudemans) and Cunaxa setirostris (Hermann) were also found. The study showed that weather factors affected mites similarly in different fruit zones of Punjab. There was a negative correlation between mite abundance and rainfall at all locations. The families viz. Tenuipalpidae, Tarsonemidae and Cunaxidae exhibited a negative correlation with temperature and a positive with maximum relative humidity (RH). Tetranychidae had positive relationship with temperature and a negative one with maximum RH. Mite species peaked differently during various months but were more abundant in the arid (Abohar) followed by central (Ludhiana) and submontane zone (Hoshiarpur) of Punjab.Keywords
Brevipalpus californicus, B. phoenicis, correlation, Cunaxa setirostris, Euseius alstoniae, Euseius finlandicus, Eutetranychus orientalis, Tetranychus urticae, Kinnow, Population Dynamics, Weather Factors.Full Text
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